Amino Acid Analyzer
A blood test for amino acids analyses your amino acid profile, which is a reliable predictor of your general nutritional health.
Techniques for Detecting:
Currently, ninhydrin and o-phthalaldehyde are the two main reagent types that can be used to analyze amino acids. The former is used to detect visual absorption, whereas the latter is used to detect fluorescence. Cation exchange chromatography is the technique for separation that is most frequently utilized.
Amino Acid Chromatography's Purpose:
The goal of this experiment is to identify individual, unidentified amino acids as well as the individual amino acids that make up several typical proteins after they are hydrolyzed. By contrasting the RF values of the unknown amino acid with those of known amino acids, this identification is done.
A protein’s relative amino acid makeup can be ascertained by AA analysis. If the amino acid sequence of a protein or peptide is known, it is also advised to calculate the absolute amount of the substance. Additionally, it plays a critical role in determining the extinction coefficient of an interest protein or antibody.
Basics of Amino Acid Analyzer:
Ion exchange liquid chromatography-based amino acid analysis is a method utilized in a variety of application fields to provide both qualitative and quantitative compositional analysis. The continuous flow chromatography method created by Spackman, Moore, and Stein in 1958 serves as the fundamental operating principle.
A separation column, a set of buffers, and a list of the amino acids to be separated from one another and any potential or distracting compounds present in the samples define an application for an amino acid analyzer.
Amino Acid Uses:
Amino acids are used by the human body to create proteins that aid in: