Transmission electron microscopy (TEM)

Are microscopes that view samples and create a vastly expanded image through the use of an electron particle beam. TEMs can magnify objects approximately 2 million times.

TEMs Working Principle:

In addition to making an image, TEMs need a high voltage beam of electrons. An electron emitter just at the head of a TEM releases electrons that go through the vacuum tube of the microscope. Instead of a single-lens reflex to focus the light, the TEM utilizes an electro-magnetic lens to concentrate the electrons through an extremely tiny beam. This beam then travels into the very thin specimen, and the electrons can deflect or strike a light source at the bottom of the microscope.

Preparation of Specimens:

A tiny enough TEM sample can transfer more electrons to generate an image with reduced power loss. As a result, sample preparation is a necessary feature of TEM analysis. A standard sequence of preparation processes for most flexible electronics includes acoustic disk slicing, puffiness, and ion milling. Slight discoloration is a preparatory process that results in a sample with a reduced center of the image and a thick enough outside edge to facilitate ease manipulation. Traditionally, particle grinding is the ultimate stage of sample processing. The use of high – voltage power pushes ionized argon atoms to the sample surface throughout this procedure. As a result of molecular diffusion, ionic bombardment on the sample surface material removal.


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